Journal: Oncology Letters

Article Title: Influence of ezrin-shRNA in combination with HSP70 on the apoptosis and proliferation of osteosarcoma cells

doi: 10.3892/ol.2016.5103

Figure Lengend Snippet: mRNA and protein expression of ezrin and HSP70 in transfected LM8 cells were detected by (A) reverse transcription-quantitative polymerase chain reaction and (B) western blotting, respectively. (C) Cellular apoptosis was analyzed by detecting the number of early and late apoptotic cells, as well as normal cells, in the NC, ezrin-shRNA and ezrin-shRNA/HSP70 groups. (D) Apoptosis analysis of stably transfected LM8 cells was performed by flow cytometry. (E) Apoptosis-associated proteins were detected by western blotting. NC, negative control; mRNA, messenger RNA; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; shRNA, small hairpin RNA; HSP70, heat shock protein 70; Bcl-2, B-cell lymphoma-2; Bax, Bcl-2 associated X protein; PI, propidium iodide; FITC, fluorescein isothiocyanate.

Article Snippet: The murine osteosarcoma cell line LM8 was purchased from the China Center for Type Culture Collection (Wuhan, China), and was cultured in Dulbecco's modified Eagle medium (DMEM; HyClone; GE Healthcare Life Sciences, Logan, UT, USA) supplemented with 10% fetal bovine serum (HyClone; GE Healthcare Life Sciences) at 37°C in a 5% CO 2 incubator.

Techniques: Expressing, Transfection, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, shRNA, Stable Transfection, Flow Cytometry, Negative Control

Journal: Oncology Letters

Article Title: Influence of ezrin-shRNA in combination with HSP70 on the apoptosis and proliferation of osteosarcoma cells

doi: 10.3892/ol.2016.5103

Figure Lengend Snippet: MTT analysis of stably transfected LM8 cells. Cell proliferation was analyzed for 7 days by MTT assay in the negative control, ezrin-shRNA and ezrin-shRNA/HSP70 groups. The absorbance of cells at day 0 was considered as 100%. shRNA, small hairpin RNA; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; HSP70, heat shock protein 70.

Article Snippet: The murine osteosarcoma cell line LM8 was purchased from the China Center for Type Culture Collection (Wuhan, China), and was cultured in Dulbecco's modified Eagle medium (DMEM; HyClone; GE Healthcare Life Sciences, Logan, UT, USA) supplemented with 10% fetal bovine serum (HyClone; GE Healthcare Life Sciences) at 37°C in a 5% CO 2 incubator.

Techniques: Stable Transfection, Transfection, MTT Assay, Negative Control, shRNA

Journal: Oncology Letters

Article Title: Influence of ezrin-shRNA in combination with HSP70 on the apoptosis and proliferation of osteosarcoma cells

doi: 10.3892/ol.2016.5103

Figure Lengend Snippet: Cytotoxic effect of HSP70-induced CTL activity on LM8 cells. The E:T ratios of 100:1, 50:1 and 25:1 were used, and the killing effect of HSP70-induced CTL activity on LM8 osteosarcoma cells was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The data is presented as the mean ± standard deviation. CTL, cytotoxic T lymphocyte; HSP70, heat shock protein 70; IL-2, interleukin-2; E, effector cells; T, target cells.

Article Snippet: The murine osteosarcoma cell line LM8 was purchased from the China Center for Type Culture Collection (Wuhan, China), and was cultured in Dulbecco's modified Eagle medium (DMEM; HyClone; GE Healthcare Life Sciences, Logan, UT, USA) supplemented with 10% fetal bovine serum (HyClone; GE Healthcare Life Sciences) at 37°C in a 5% CO 2 incubator.

Techniques: Activity Assay, Standard Deviation

Journal: Oncology Letters

Article Title: Influence of ezrin-shRNA in combination with HSP70 on the apoptosis and proliferation of osteosarcoma cells

doi: 10.3892/ol.2016.5103

Figure Lengend Snippet: Tumor formation and characterization upon injected LM8 cells were transfected with different vectors. (A) Tumor growth in mice injected with stably transfected LM8 cells. (B) Tumor weight in mice 6 weeks after injection. (C) Image of tumors in mice (magnification, ×100). (D) Histopathological changes of tumors from tumor-bearing mice (magnification, ×100). Tumors were obtained from mice in the negative control, ezrin-shRNA and ezrin-shRNA/HSP70 (dual) groups 6 weeks after injection, and subjected to histopathological analysis using hematoxylin and eosin staining. shRNA, small hairpin RNA; HSP70, heat shock protein 70.

Article Snippet: The murine osteosarcoma cell line LM8 was purchased from the China Center for Type Culture Collection (Wuhan, China), and was cultured in Dulbecco's modified Eagle medium (DMEM; HyClone; GE Healthcare Life Sciences, Logan, UT, USA) supplemented with 10% fetal bovine serum (HyClone; GE Healthcare Life Sciences) at 37°C in a 5% CO 2 incubator.

Techniques: Injection, Transfection, Stable Transfection, Negative Control, shRNA, Staining

Journal: Cancer Medicine

Article Title: Hybrid liposomes inhibit tumor growth and lung metastasis of murine osteosarcoma cells

doi: 10.1002/cam4.67

Figure Lengend Snippet: Inhibitory effects of HL-23 on the lung metastasis of LM8 cells in homograft mouse models in vivo. (a) Micrographs of lung tissue resected from the mouse models treated with HL-23 for 14 days by HE staining method. Scale bars: 500 μm. (b) Dimensions of metastatic tumor in the lung tissue of mouse models treated with HL-23 for 14 days. Error bars indicate SE ( n = 5 per group). * P < 0.05 to control. Control: 5% glucose solution, DMPC liposomes: 203 mg/kg/day, HL-23: 203 mg/kg/day for DMPC, 41 mg/kg/day for C 12 (EO) 23 . HL, hybrid liposomes; LM8, murine osteosarcoma; HE, hematoxylin and eosin; SE, standard error; DMPC, l -α-dimyristoylphosphatidylcholine; C 12 (EO) 23 , polyoxyethylene(23) dodecyl ether.

Article Snippet: Metastatic murine osteosarcoma cell line (LM8) was obtained in October 2011 from the cell bank Riken Bioresource Center (Saitama, Japan) [ ] and frozen as original stocks in October 2011.

Techniques: In Vivo, Staining, Control, Liposomes

Journal: Molecules

Article Title: Nanohydroxyapatite-Mediated Imatinib Delivery for Specific Anticancer Applications

doi: 10.3390/molecules25204602

Figure Lengend Snippet: TOX-8 viability test results for NI-1 ( A – C ), L929 ( D – F ), and D17 ( G – I ) cells depending on the concentration of experimental compounds used: imatinib, nHAp modified with imatinib and nHAp alone. IC 50 is indicated if possible. R 2 stands for the coefficient of determination.

Article Snippet: The canine osteosarcoma D17 control cell line (European Collection of Authenticated Cell Cultures, ECACC) was cultured in modified Eagle’s medium (MEM) supplemented with F12 Ham’s nutrient an 10% FBS.

Techniques: Concentration Assay, Modification

Journal: Molecules

Article Title: Nanohydroxyapatite-Mediated Imatinib Delivery for Specific Anticancer Applications

doi: 10.3390/molecules25204602

Figure Lengend Snippet: Comparison of results of cytotoxicity analysis of imatinib and modified nHAp on NI-1 ( A ), L929 ( B ), and D17 ( C ) cells. Results are expressed as mean ± SD, * p < 0.05, ** p < 0.01.

Article Snippet: The canine osteosarcoma D17 control cell line (European Collection of Authenticated Cell Cultures, ECACC) was cultured in modified Eagle’s medium (MEM) supplemented with F12 Ham’s nutrient an 10% FBS.

Techniques: Comparison, Modification